Former ZH-T-type T-shaped maze video analysis system First, Principle nearly half a century, Kivy and Dember et al. Demonstrated in rats can identify changes in color T- shaped maze arms (T-maze). They found that the male rats were placed in the trunk T- shaped maze arm 15-30min, let it be able to see, but can not enter the black and white arms. Then, change the color of which one arm, both arms with black or white. Let rat free choice T- shaped arm. The results showed that rats always choose to change the color of the arm (arm novel). This process relies on animal memory to complete. Thus evolved T- shaped maze become one of the most commonly used animal models currently used to evaluate spatial memory. Of course, now the T- shaped maze using food colors instead of the arm as a power animal inquiry. This is usually an animal model to study spatial working memory (spatial working memory), that the animal was measured only in the current period of useful information operations. After improving T- shaped maze also be used to evaluate the reference memory (reference memory), i.e., on any day in this experiment, once a test has any use of the information recording. T-maze is to detect spatial working memory in rodents, a classic behavioral methods associated with the prefrontal cortex function. Mouse Diet to 85% of the original weight. Technical parameters: 1, the total time of the experiment 2, 3 total distance events, the left arm into the number 4, enter the number 5 right arm, left arm incubation period (from left arm into the main boom time) 6, the right arm incubation period (enter from the main arm the right arm of the time) 7, the main arm of the residence time of 8, the main arm activity from a) an open field to select a variety of geometric shapes, can be easily sketched out a variety of open field shapes; 2) support for offline and online real-time analysis, saving experiment Time and laboratory personnel 3) automatic tracking software analysis, generate EXCEL file can be directly analyzed with SPSS statistical analysis software; (software also comes with powerful analysis, contains more than 30 kinds of statistical tests) 4) can record more artificial up to 26 kinds of video software can not detect the phenomenon, such as the number of standing, grooming, fecal pellets, the number of hops, etc. 5) video capture have the original video, ensure that the test to be investigated; and (software can choose to save the original recording, you can choose not to save the video to save hard disk space) 6) experimental process automation, avoiding artificial observation, counting subjective error and interference introduced to the experimental animals; ① experimental methods before training 2 d, touch mouse and place it in T-maze accommodate 10 min, in the maze arms into the food bowl, until after the completion of the mice fed it out. ② training experiment: Each trial consists of two parts, namely the forced run and test run. forced run: choose T maze arm with a wooden gate will be closed, the mouse can only get food into the arm. Feeding is completed, remove immediately cleaned with alcohol to remove the odor. After 15 s, removed all the gates. Test run: arms fully open, the mice were placed in the beginning of the arm, if the mouse choose not to enter the arm, for the correct choice, to give food reward; if re-entering the same arm, was the wrong choice, do not give food. Each animal day 4 trial, each time interval 15min, continuous 4 d, respectively, to calculate the correct rate for each group of mice a day. When the average accuracy rate of more than 85%, ending the training phase. ③ test method T maze test: forced run and extend the time interval between test run and to 1min 3min, were calculated for each group of mice accuracy.