Former ZH-T-type T-shaped maze video analysis system Principle of nearly half a century, Kivy and Dember et al. Demonstrated in rats can distinguish the color change arms T- shaped maze (T-maze). They found that male rats were placed in the trunk T- shaped maze arm 15-30min, let it be able to see, but can not enter the black and white arms. Then, change the color in which one arm, both arms with black or white. Rats are free to choose T- shaped arm. The results showed that rats always choose to change the color of the arm (arm novel). This process relies on the animal's memory to complete. Thus evolved T- shaped maze to become one of the most commonly used animal models currently used to evaluate spatial memory. Of course, now the T- shaped maze using color food rather than as a power animal arm inquiry. Usually we use this model to study animal spatial working memory (spatial working memory), that is, to determine an animal only in the current period of useful information operations. After the improvement of the T- shaped labyrinth can also be used to evaluate the reference memory (reference memory), that is, in this experiment, any day, any time the test has used the information recording. T maze is to detect spatial working memory in rodents, a classic behavioral methods associated with the prefrontal cortex function. Mouse diet to 85% of the original weight. Technical parameters: 1, the total time of the experiment 2, 3 total distance events, the left arm into the number 4, enter the number 5 right arm, left arm latency (into the left arm from the main arm time) 6, the right arm latency (accessed from the main arm the right arm of the time) 7, the main arm retention time of 8, the main arm activity from 1) a variety of geometric shapes choose open field, can be easily sketched out a variety of open field shapes; 2) support for offline and online real-time analysis, saving experiment time and laboratory personnel 3) software to automatically trace analysis, generate EXCEL file can be directly analyzed in SPSS statistical analysis software; (software also comes with powerful analysis, containing more than 30 kinds of statistical tests) 4) can record more artificial up to 26 kinds of video software can not detect the phenomenon, such as the number of standing, grooming, fecal pellets, the number of hops, etc. 5) Video capture has the original recording, ensure that the test can be found; and (software can choose to save the original recording, you can choose not to save the video to save hard disk space) 6) test process automation, to avoid human observation, counting subjective error and interference introduced in experimental animals; the first experimental method ① training 2 d, touch mouse and place it in T maze adaptation 10 min, and the maze arms into a bowl of food, feeding the mice will be completed to remove it. ② training experiment: Each trial consists of two parts, namely forced run and test run. forced run: choose T maze arm with a wooden gate will be closed, the mouse can only get food into the arm. Feeding is complete, remove immediately cleaned with alcohol to remove odor. After 15 s, removed all the gates. Test run: arms fully open, the mice were placed in the beginning of the arm, if not into the arms mouse to select for the correct choice, to give a food reward; if re-enter the same arm, was the wrong choice, do not give food. Each animal per day 4 trial, each time interval 15min, continuous 4 d, each group of mice were calculated correct rate per day. When the average accuracy rate of more than 85%, the end of the training phase. T-maze test method ③ experiment: prolonged forced run and test run to 1min and interval between 3min, each group of mice were calculated accuracy.